LOCUS       sequence-112632-         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT
            promotes cellular uptake of recombinant Cre into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F
  TITLE     Ability of the hydrophobic FGF and basic TAT peptides to promote 
            cellular uptake of recombinant Cre recombinase: a tool for efficient
            genetic engineering of mammalian genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.
  PUBMED    11904364
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 2. 99(7):4489-94."
COMMENT     Sequence Label: pTriEx-HTNC
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(195..212)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(233..342)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    358..382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(382..401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(383..401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(443..462)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(524..546)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg
       61 accggtaatg caggcaaatt ttggtgtacg gtcagtaaat tggacacctt cctcttcttc
      121 ttcaagctta cactagtgcc tgcaggcgga cggcgacgtt ggcgacgttt cttgcgacct
      181 gcagcgccca tgccgtgatg gtgatggtga tggcccatgg tatatctcct ttgattgtaa
      241 ataaaatgta atttacagta tagtatttta attaatatac aaatgatttg ataataattc
      301 ttatttaact ataatatatt gtgttgggtt gaattaaagg tcccggatta actccgggga
      361 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcggtc cgatccaatt
      421 ctttgccaaa atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa
      481 agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc
      541 cgaaccccgc cctgccccgt cccccccgaa ggcagc
//
