LOCUS       sequence-112641-       14926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human p110 alpha with E545K mutation in avian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Kang S, Bader AG, Vogt PK
  TITLE     Phosphatidylinositol 3-kinase mutations identified in human cancer 
            are oncogenic.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):802-7. Epub 2005 Jan 
            12.
  PUBMED    15647370
REFERENCE   2  (bases 1 to 14926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-01-18"; volume: "102(3)"; pages: 
            "802-7. Epub 2005 Jan 12"
COMMENT     Sequence Label: RCAS(A) human p110 alpha E545K
FEATURES             Location/Qualifiers
     source          1..14926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..403
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(994..1016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1005..1021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1029..1035,1036..1053,1054..1059))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1102..1120)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1188..1292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1293..1361,1362..2153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1511..1530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2324..2912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2813..2832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3066..3083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3098..3238
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3164..3183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3324..3346
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3340..3531)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        4376..4637
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 cggcccgatg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta
       61 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc ccttaggata tagtagtttc
      121 gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      181 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      241 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      301 gattggacga accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga
      361 tacaataaac gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac
      421 cgttgattcc ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc
      481 cgacgtgata gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc
      541 cgtctcgtct atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga
      601 ggcagaagct gagtaccgtc ggaggggatc cacaggacgg gtgtggtcgc catgatcgcg
      661 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      721 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
      781 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
      841 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
      901 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
      961 ctaccgcatt aaagcttcca aacttggctg tttcctgtgt gaaattgtta tccgctcaca
     1021 attccacaca ttatacgagc cggaagcata aagtgtaaaa cctggggtgc ctaatgagtg
     1081 agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1141 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1261 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1321 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1381 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1441 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1501 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     1561 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1621 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1681 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1741 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1801 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     1861 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1921 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1981 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2041 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2101 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2161 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2221 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2461 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2941 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3001 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3061 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3121 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3181 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3241 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3301 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3361 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     3421 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     3481 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca cttgatgcct
     3541 ccgtgtaagg gggaatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     3601 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     3661 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     3721 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     3781 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     3841 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     3901 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     3961 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     4021 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     4081 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     4141 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     4201 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gatgcgatgt acgggccaga
     4261 tatacgcgta tctgagggga ctagggtgtg tttaggcgaa aagcggggct tcggttgtac
     4321 gcggttagga gtccccttag gatatagtag tttcgctttt gcatagggag ggggaaatgt
     4381 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     4441 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     4501 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattccgca
     4561 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt tgaccattca
     4621 ccacattggt gtgcacctgg gttgatggcc ggaccgttga ttccctgacg actacgagca
     4681 cctgcatgaa gcagaaggct tcatttggtg accccgacgt gatagttagg gaatagtggt
     4741 cggccacaga cggcgtggcg atcctgtctc catccgtctc gtctatcggg aggcgagttc
     4801 gatgaccctg gtggaggggg ctgcggctta gggaggcaga agctgagtac cgtcggaggg
     4861 agctccaggg cccggagcga ctgacccctg ccgagaactc agagggtcgt cggaagacgg
     4921 agagtgagcc cgacgaccac cccaggcacg tctttggtcg gcctgcggat caagcatgga
     4981 agccgtcatt aaggtgattt cgtccgcgtg taaaacctat tgcgggaaaa tctctccttc
     5041 taagaaggaa ataggggcca tgttgtccct gttacaaaag gaagggttgc ttatgtctcc
     5101 ctcagattta tattctccgg ggtcctggga tcccatcact gcggcgctct cccagcgggc
     5161 aatggtactt ggaaaatcgg gagagttaaa aacctgggga ttggttttgg gggcattgaa
     5221 ggcggctcga gaggaacagg ttacatctga gcaagcaaag ttttggttgg gattaggggg
     5281 agggagggtc tctcccccag gtccggagtg catcgagaaa ccagctacgg agcggcgaat
     5341 cgacaaaggg gaggaggtgg gagaaacaac tgtgcagcga gatgcgaaga tggcgccaga
     5401 ggaagcggcc acacctaaaa ccgttggcac atcctgctat cattgcggaa cagctgttgg
     5461 ctgcaattgc gccaccgcca cagcctcggc ccctcctccc ccttatgtgg ggagtggttt
     5521 gtatccttcc ctggcggggg tgggagagca gcagggccag ggagataaca cgtctcgggg
     5581 ggcggagcag ccaagggagg agccagggca cgcgggtcag gcccctgggc cggccctgac
     5641 tgactgggca agggtaaggg aggagcttgc gagtactggt ccgcccgtgg tggccatgcc
     5701 tgtagtgatt aagacagagg gacccgcctg gacccctctg gagccaaaat tgatcacaag
     5761 actggctgat acggtcagga ccaagggctt acgatccccg atcactatgg cagaagtgga
     5821 agcgctcatg tcctccccgt tgctgccgca tgacgtcacg aatctaatga gagtgatttt
     5881 aggacctgcc ccatatgcct tatggatgga cgcttgggga gtccaactcc agacggttat
     5941 agcggcagcc actcgcgacc cccgacaccc agcgaacggt caagggcggg gggaacggac
     6001 taacttggat cgattaaagg gcttagctga tgggatggtg ggcaacccac agggtcaggc
     6061 cgcattatta agaccggggg aattggttgc tattacggcg tcggctctcc aggcgtttag
     6121 agaagttgcc cggctggcgg aacctgcagg tccatgggcg gacatcacgc agggaccatc
     6181 tgagtccttt gttgattttg ccaatcggct tataaaggcg gttgaggggt cagatctccc
     6241 gccttccgcg cgggctccgg tgatcattga ctgctttagg cagaagtcac agccagatat
     6301 tcagcagctt atacgggcag caccctccac gctgaccacc ccaggagaga taatcaaata
     6361 tgtgctagac aggcagaaga ttgcccctct tacggatcaa ggcatagccg cggccatgtc
     6421 gtctgctatc cagcccttag ttatggcagt agtcaataga gagagggatg gacaaactgg
     6481 gtcgggtggt cgtgcccgag ggctctgcta cacttgtgga tccccgggac attatcaggc
     6541 acagtgcccg aaaaaacgaa agtcaggaaa cagccgtgag cgatgtcagc tgtgtgacgg
     6601 gatgggacac aacgctaaac agtgtaggaa gcgggatggc aaccagggcc aacgcccagg
     6661 aagaggtctc tcttcggggc cgtggcccgg ccctgagcag cctgccgtct cgttagcgat
     6721 gacaatggaa cataaagatc gccccttggt tagggtcatt ctgactaaca ctgggagtca
     6781 tccagtcaaa caacgttcgg tgtatatcac cgcgctgttg gactccggag cggacatcac
     6841 tattatttcg gaggaggatt ggcctactga ttggccggtg gtggacaccg cgaacccaca
     6901 gatccatggc ataggagggg gaattcccat gcgaaaatcc cgggatatga tagaggtggg
     6961 ggttattaac cgagacgggt cgttggagcg acccctgctc ctcttccccg cagtcgctat
     7021 ggttagaggg agtatcctag gaagagattg tctgcagggc ctagggctcc gcttgacaaa
     7081 tttataggga gggccactgt tcttactgtt gcgctacatc tggctattcc gctcaaatgg
     7141 aagtcagacc gcacgcctgt gtggattgac cagtggcccc ttcctgaagg taaacttgta
     7201 gcgctaacgc aattagtgga aaaagaatta cagttaggac atatagagcc ctcacttagt
     7261 tgttggaaca cacctgtttt tcgtgatccg gaaggcttcc gggtcttatc gcttattgca
     7321 tgatttgcgc gctgttaacg ccaagcttgt cccttttggg gccgtccaac agggggcgcc
     7381 agttctctcc gcgctcccgc gtggctggcc cctgatggtc ctagacctca aggattgctt
     7441 cttttctatc cctcttgcgg aacaagatcg cgaagctttt gcatttacgc tcccctctgt
     7501 gaataaccag gcccccgctc gaagattcca atggaaggtc ttgccccaag ggatgacctg
     7561 ttctcccact atctgtcagt tggtagtggg tcaggtgctc gagcccttgc gactcaagca
     7621 cccagctctg cgcatgttgc attatatgga cgatcttttg ctagccgcct caagtcatga
     7681 tgggttggaa gcggcaggga aggaggttat cggtacattg gaaagagccg ggttcactat
     7741 ttcgccggat aagatccaga gggagcccgg agtacaatat cttgggtaca agttaggcag
     7801 tacgtatgta gcacccgtag gcttggtagc agaacccagg atagccacct tgtgggatgt
     7861 tcaaaagctg gtggggtcac ttcagtggct tcgcccagcg ttagggatcc cgccacgact
     7921 gatgggtccc ttttatgagc agttacgagg gtcagatcct aacgaggcga gggaatggaa
     7981 tctagacatg aaaatggcct ggagagagat cgtacagctt agcactactg ctgccttgga
     8041 acgatgggac cctgcccagc ctctggaagg agcggtcgct agatgtgaac agggggcaat
     8101 aggggtcctg ggacagggac tgtccacaca cccaaggcca tgtttgtggt tattctccac
     8161 ccaacccacc aaggcgttta ctgcttggtt agaagtgctc acccttttga ttactaagct
     8221 acgcgcttcg gcagtgcgaa cctttggcaa ggaggttgat atcctcctgt tgcctgcatg
     8281 cttccgggag gaccttccgc tcccggaggg gatcctgtta gcacttaggg ggtttgcagg
     8341 aaaaatcagg agtagtgaca cgccatctat ttttgacatt gcgcgtccac tgcatgtttc
     8401 tctgaaagtg agggttaccg accaccctgt gccgggaccc actgtcttta ccgacgcctc
     8461 ctcaagcacc cataaagggg tggtagtctg gagggagggc ccaaggtggg agataaaaga
     8521 aatagttgat ttgggggcaa gtgtacaaca actggaggca cgcgctgtgg ccatggcact
     8581 tctgctgtgg ccgacaacgc ccactaatgt agtgactgac tctgcgtttg ttgcgaaaat
     8641 gttactcaag atgggacagg agggagtccc gtctacagcg gcagctttta ttttagagga
     8701 tgcgttaagc caaaggtcag ccatggccgc cgttctccac gtgcggagtc attctgaagt
     8761 gccagggttt ttcacagaag gaaatgacgt ggcagatagc caagccacct ttcaagcgta
     8821 tcccttgaga gaggctaaag atcttcatac cgctctccat attggacccc gcgcgctatc
     8881 caaagcgtgt aatatatcta tgcagcaggc tagggaggtt gttcagacct gcccgcattg
     8941 taattcagcc cctgcgttgg aggccggggt aaaccctagg ggtttgggac ccctacagat
     9001 atggcagaca gactttacgc ttgagcctag aatggctccc cgttcctggc tcgctgttac
     9061 tgtggacacc gcctcatcag cgatagtcgt aactcagcat ggccgtgtta catcggttgc
     9121 tgcacaacat cattgggcca cggctatcgc cgttttggga agaccaaagg ccataaaaac
     9181 agataacggg tcctgcttca cgtctagatc cacgcgggag tggctcgcga gatgggggat
     9241 agcacacacc accgggattc cgggaaattc ccagggtcaa gctatggtag agcgggccaa
     9301 ccggctcctg aaagataaga tccgtgtgct tgcggagggg gacggcttta tgaaaagaat
     9361 ccccgccagc aaacaggggg aactactagc caaggcaatg tatgccctca atcactttga
     9421 gcgtggtgaa aacacaaaaa caccggtaca aaaacactgg agacctaccg ttcttacaga
     9481 aggacccccg gttaaaatac gaatagagac aggggagtgg gaaaaaggat ggaacgtgct
     9541 ggtctgggga cgaggttatg ccgctgtgaa aaacagggac actgataagg ttatttgggt
     9601 accctctcgg aaagttaaac cggatgtcac ccaaaaggat gaggtgacta agaaagatga
     9661 ggcgagccct ctttttgcag gcatttctga ctggataccc tgggaagacg agcaagaagg
     9721 actccaagga gaaaccgcta gcaacaagca agaaagaccc ggagaagaca cccttgctgc
     9781 caacgagagt taattatatt ctcattattg gtgtcctggt cttgtgtgag gttacggggg
     9841 taagagctga tgtccactta ctcgagcagc cagggaacct ttggattaca tgggccaacc
     9901 gtacaggcca aacggatttt tgcctctcta cacagtcagc cacctcccct tttcaaacat
     9961 gtttgatagg tatcccgtcc cctatttccg agggtgattt taagggatat gtttctgata
    10021 caaattgcac caccttggga actgatcggt tagtctcgtc agccgacttt actggcggac
    10081 ctgacaacag taccaccctc acttatcgga aggtctcatg cttgttgtta aagctgaatg
    10141 tctctatgtg ggatgagcca cctgaactac agctgttagg ttcccagtct ctccctaaca
    10201 ttactaatat tgctcagatt tccggtataa ccgggggatg cgtaggcttc agaccacaag
    10261 gggttccttg gtatctaggt tggtctagac aggaggccac gcggtttctc cttagacacc
    10321 cctctttctc taaatccacg gaaccgttta cagtggtgac agcggatagg cacaatcttt
    10381 ttatggggag tgagtactgc ggtgcatatg gctacagatt ttggaacatg tataactgct
    10441 cacaggtggg gcggcagtac cgctgtggta atgcgcgcac gccccgcacg ggtcttcctg
    10501 aaatccagtg tacaaggaga ggaggcaaat gggttaatca atcacaggaa attaatgagt
    10561 cggagccgtt cagctttacg gtgaactgta cagctagtag tttgggtaat gccagtgggt
    10621 gttgcggaaa agcaggcacg attctcccgg gaaagtgggt cgacagcaca caaggtagtt
    10681 tcaccaaacc aaaagcgcta ccacccgcaa ttttcctcat ttgtggggat cgcgcatggc
    10741 aaggaattcc cagtcgtccg gtagggggcc cctgctattt aggcaagctt accatgttag
    10801 cacctaagca tacagatatt ctcaaggtgc ttgtcaattc atcgcggaca ggtataagac
    10861 gtaaacgaag cacctcacac ctggatgata catgctcaga tgaagtgcag ctttggggtc
    10921 ctacagcaag aatctttgca tctatcctag ccccgggggt agcagctgcg caagccttaa
    10981 gagaaattga gagactagcc tgttggtccg ttaaacaggc taacttgaca acatcactcc
    11041 tcggggactt attggatgat gtcacgagta ttcgacacgc ggtcctgcag aaccgagcgg
    11101 ctattgactt cttgctccta gctcacggcc atggctgtga ggacgttgcc ggaatgtgct
    11161 gtttcaattt gagtgatcag agtgagtcta tacagaagaa gttccagcta atgaaggaac
    11221 atgtcaataa gatcggcgtg gatagcgacc taattggaag ttggctgcga ggactattcg
    11281 ggggaatagg agaatgggcc gttcatttgc tgaaaggact gcttttgggg cttgtagtta
    11341 ttttgttgct agtagtgtgc ctgccttgcc ttttgcaaat gttatgcggt aataggagaa
    11401 agatgattaa taactccatc agctaccaca cggaatataa gaagctgcaa aaggcctgtg
    11461 ggcagcctga aagcagaata gtataaggca gtacatgggt ggtggtatag cgcttgcgag
    11521 tccatcgagc aaggcaggaa agacagctat tggtaattgt gaaatacgct tttgtctgtg
    11581 tgctgcagga gctgagctga ctctgctggt ggcctcgcgt accactgtgg catcgggcca
    11641 ttacggccgc ggccgcctct agaggatccg atatcgaatt cacgcgtgcc gccatgcctc
    11701 cacgaccatc atcaggtgaa ctgtggggca tccacttgat gcccccaaga atcctagtag
    11761 aatgtttact accaaatgga atgatagtga ctttagaatg cctccgtgag gctacattaa
    11821 taaccataaa gcatgaacta tttaaagaag caagaaaata ccccctccat caacttcttc
    11881 aagatgaatc ttcttacatt ttcgtaagtg ttactcaaga agcagaaagg gaagaatttt
    11941 ttgatgaaac aagacgactt tgtgaccttc ggctttttca acccttttta aaagtaattg
    12001 aaccagtagg caaccgtgaa gaaaagatcc tcaatcgaga aattggtttt gctatcggca
    12061 tgccagtgtg tgaatttgat atggttaaag atccagaagt acaggacttc cgaagaaata
    12121 ttctgaacgt ttgtaaagaa gctgtggatc ttagggacct caattcacct catagtagag
    12181 caatgtatgt ctatcctcca aatgtagaat cttcaccaga attgccaaag cacatatata
    12241 ataaattaga taaagggcaa ataatagtgg tgatctgggt aatagtttct ccaaataatg
    12301 acaagcagaa gtatactctg aaaatcaacc atgactgtgt accagaacaa gtaattgctg
    12361 aagcaatcag gaaaaaaact cgaagtatgt tgctatcctc tgaacaacta aaactctgtg
    12421 ttttagaata tcagggcaag tatattttaa aagtgtgtgg atgtgatgaa tacttcctag
    12481 aaaaatatcc tctgagtcag tataagtata taagaagctg tataatgctt gggaggatgc
    12541 ccaatttgat gttgatggct aaagaaagcc tttattctca actgccaatg gactgtttta
    12601 caatgccatc ttattccaga cgcatttcca cagctacacc atatatgaat ggagaaacat
    12661 ctacaaaatc cctttgggtt ataaatagtg cactcagaat aaaaattctt tgtgcaacct
    12721 acgtgaatgt aaatattcga gacattgata agatctatgt tcgaacaggt atctaccatg
    12781 gaggagaacc cttatgtgac aatgtgaaca ctcaaagagt accttgttcc aatcccaggt
    12841 ggaatgaatg gctgaattat gatatataca ttcctgatct tcctcgtgct gctcgacttt
    12901 gcctttccat ttgctctgtt aaaggccgaa agggtgctaa agaggaacac tgtccattgg
    12961 catggggaaa tataaacttg tttgattaca cagacactct agtatctgga aaaatggctt
    13021 tgaatctttg gccagtacct catggattag aagatttgct gaaccctatt ggtgttactg
    13081 gatcaaatcc aaataaagaa actccatgct tagagttgga gtttgactgg ttcagcagtg
    13141 tggtaaagtt cccagatatg tcagtgattg aagagcatgc caattggtct gtatcccgag
    13201 aagcaggatt tagctattcc cacgcaggac tgagtaacag actagctaga gacaatgaat
    13261 taagggaaaa tgacaaagaa cagctcaaag caatttctac acgagatcct ctctctgaaa
    13321 tcactaagca ggagaaagat tttctatgga gtcacagaca ctattgtgta actatccccg
    13381 aaattctacc caaattgctt ctgtctgtta aatggaattc tagagatgaa gtagcccaga
    13441 tgtattgctt ggtaaaagat tggcctccaa tcaaacctga acaggctatg gaacttctgg
    13501 actgtaatta cccagatcct atggttcgag gttttgctgt tcggtgcttg gaaaaatatt
    13561 taacagatga caaactttct cagtatttaa ttcagctagt acaggtccta aaatatgaac
    13621 aatatttgga taacttgctt gtgagatttt tactgaagaa agcattgact aatcaaagga
    13681 ttgggcactt tttcttttgg catttaaaat ctgagatgca caataaaaca gttagccaga
    13741 ggtttggcct gcttttggag tcctattgtc gtgcatgtgg gatgtatttg aagcacctga
    13801 ataggcaagt cgaggcaatg gaaaagctca ttaacttaac tgacattctc aaacaggaga
    13861 agaaggatga aacacaaaag gtacagatga agtttttagt tgagcaaatg aggcgaccag
    13921 atttcatgga tgctctacag ggctttctgt ctcctctaaa ccctgctcat caactaggaa
    13981 acctcaggct tgaagagtgt cgaattatgt cctctgcaaa aaggccactg tggttgaatt
    14041 gggagaaccc agacatcatg tcagagttac tgtttcagaa caatgagatc atctttaaaa
    14101 atggggatga tttacggcaa gatatgctaa cacttcaaat tattcgtatt atggaaaata
    14161 tctggcaaaa tcaaggtctt gatcttcgaa tgttacctta tggttgtctg tcaatcggtg
    14221 actgtgtggg acttattgag gtggtgcgaa attctcacac tattatgcaa attcagtgca
    14281 aaggcggctt gaaaggtgca ctgcagttca acagccacac actacatcag tggctcaaag
    14341 acaagaacaa aggagaaata tatgatgcag ccattgacct gtttacacgt tcatgtgctg
    14401 gatactgtgt agctaccttc attttgggaa ttggagatcg tcacaatagt aacatcatgg
    14461 tgaaagacga tggacaactg tttcatatag attttggaca ctttttggat cacaagaaga
    14521 aaaaatttgg ttataaacga gaacgtgtgc catttgtttt gacacaggat ttcttaatag
    14581 tgattagtaa aggagcccaa gaatgcacaa agacaagaga atttgagagg tttcaggaga
    14641 tgtgttacaa ggcttatcta gctattcgac agcatgccaa tctcttcata aatcttttct
    14701 caatgatgct tggctctgga atgccagaac tacaatcttt tgatgacatt gcatacattc
    14761 gaaagaccct agccttagat aaaactgagc aagaggcttt ggagtatttc atgaaacaaa
    14821 tgaatgatgc acatcatggt ggctggacaa caaaaatgga ttggatcttc cacacaatta
    14881 aacagcatgc attgaactga atcgatgtcg acgggcccgg ccgcct
//
