LOCUS       sequence-112646-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2, URA3 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLUM (version 2)
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..43)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     143..162
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        309..496
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
       61 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcgga
      121 cgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataaa
      181 ttcccgtttt aagagcttgg tgagcgctag gagtcactgc caggtatcgt ttgaacacgg
      241 cattagtcag ggaagtcata acacagtcct ttcccgcaat tttctttttc tattactctt
      301 ggcctcctct agtacactct atattttttt atgcctcggt aatgattttc attttttttt
      361 ttccacctag cggatgactc tttttttttc ttagcgattg gcattatcac ataatgaatt
      421 atacattata taaagtaatg tgatttcttc gaagaatata ctaaaaaatg agcaggcaag
      481 ataaacgaag gcaaagatga cagagcagaa agccctagta aagcgtatta caaatgaaac
      541 caagattcag attgcgatct ctttaaaggg tggtccccta gcgatagagc actcgatctt
      601 cccagaaaaa gaggcagaag cagtagcaga acaggccaca caatcgcaag tgattaacgt
      661 ccacacaggt atagggtttc tggaccatat gatacatgct ctggccaagc attccggctg
      721 gtcgctaatc gttgagtgca ttggtgactt acacatagac gaccatcaca ccactgaaga
      781 ctgcgggatt gctctcggtc aagcttttaa agaggcccta ggggccgtgc gtggagtaaa
      841 aaggtttgga tcaggatttg cgcctttgga tgaggcactt tccagagcgg tggtagatct
      901 ttcgaacagg ccgtacgcag ttgtcgaact tggtttgcaa agggagaaag taggagatct
      961 ctcttgcgag atgatcccgc attttcttga aagctttgca gaggctagca gaattacc
//
