LOCUS       sequence-112661-        2970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. solfataricus PCNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Hirakawa H, Nagamune T
  TITLE     Molecular assembly of P450 with ferredoxin and ferredoxin reductase 
            by fusion to PCNA.
  JOURNAL   Chembiochem. 2010 Jul 26;11(11):1517-20. doi: 
            10.1002/cbic.201000226.
  PUBMED    20607777
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cbic"; 
            journalName: "Chembiochem"; date: "2010-07-26- 26"; volume: "11"; 
            issue: "11"; pages: "1517-20"
COMMENT     Sequence Label: pHSG-PCNA1
FEATURES             Location/Qualifiers
     source          1..2970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..192
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             193..852
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(259..278)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1060..1081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1096..1101,1102..1119,1120..1126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1134..1150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1139..1161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1158..1174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1158..1174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1987..2004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1987..2003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1996..2018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2158..2175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2329..2917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2409..2428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
       61 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      121 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      181 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      241 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      301 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      361 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      421 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      481 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      541 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      601 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      661 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      721 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
      781 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      841 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
      901 gaataagtga taataagcgg atgaatggca gaaattcagc ttggcccagt gccaagctcc
      961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc atatgtttaa
     1201 gattgtttac cctaatgcaa aagacttttt ctcttttata aattccatta cgaacgttac
     1261 tgattccatt attctaaatt tcactgaaga tggaatattt tcgagacatt tgactgaaga
     1321 taaagtgcta atggcgatta tgagaatacc taaagatgtt ttaagcgaat atagtattga
     1381 tagccctact tcagttaaat tggatgtctc ttcagtaaag aagattcttt caaaagccag
     1441 ctcaaagaaa gctacgatcg agttgacaga gactgatagt ggattaaaga ttataataag
     1501 agatgagaag agtggggcga aaagtacaat ttatattaaa gcagagaaag gtcaagttga
     1561 acaactaact gaaccgaaag ttaatttggc cgtgaatttc actacagatg aaagtgtact
     1621 taatgtaata gcagctgacg taactttggt tggagaagag atgaggattt caacagagga
     1681 ggataagata aagatagagg ctggtgagga ggggaaaaga tatgtcgcat ttcttatgaa
     1741 ggataaaccc ctaaaggaac tttctataga tacatcggca tcatcttctt atagcgcgga
     1801 aatgtttaag gatgctgtaa aaggtctaag aggtttttcg gccccaacaa tggtaagctt
     1861 tggagaaaat ctccctatga aaattgacgt agaagcagta agtgggggtc acatgatctt
     1921 ttggatagcg ccaaggttat gaagagcgga tcctctagag tcgacctgca ggcatgcaag
     1981 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2041 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2101 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgagcttctt ccgcttcctc
     2161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2521 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2761 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2881 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2941 ggggtctgac gctcagtgga actccgtcga
//
