LOCUS       sequence-112663-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes g2 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_g2gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(294..356)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(369..444)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gatgtttcac agactgtgtc aatgacttca caacatcaaa gctaaccaat aaggaacaaa
       61 catgcatcat gaagtgctca gaaaagttct tgaagcatag cgaacgtgta gggcagcgtt
      121 tccaagaaca aaacgctgcc ttgggacaag gcttgggccg ataaggtgta ctggcgtata
      181 tatatctaat tatgtatctc tggtgtagcc catttttagc atgtaaatat aaagagaaac
      241 catatctaat ctaaccaaat ccaaacaaaa ttcaatagtt actatcgctt ttttctttct
      301 gtatcgcaaa taagtgaaaa ttaaaaaaga aagattaaat tggaagttgg atatggctcg
      361 agaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta
      421 acttgctatt tctagctcta aaacctttaa ctaatacttt caacaagctt ctgccaatcg
      481 cagctcccag agtttcgttc ccagccgcga gcaccacagc gtaccatgtg cgccncgagg
      541 cctcaaacgt aaaacaatcg aaacgaaaag aaacagacta ta
//
