LOCUS       sequence-112664-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c4 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c4gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(292..354)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(367..442)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtttcacaga ctgtgtcaat gacttcacaa catcaaagct aaccaataag gaacaaacat
       61 gcatcatgaa gtgctcagaa aagttcttga agcatacgcg aacgtgtagg gcagcgtttc
      121 caagaacaaa acgctgcctt gggacaaggc ttgggccgat aaggtgtact ggcgtatata
      181 tatctaatta tgtatctctg gtgtagccca tttttagcat gtaaatataa agagaaacca
      241 tatctaatct aaccaaatcc aaacaaaatt caatagttac tatcgctttt ttctttctgt
      301 atcgcaaata agtgaaaatt aaaaaagaaa gattaaattg gaagttggat atggctcgag
      361 aaaaaagcac cgactcggtg ccactttttc aagtcgataa cggactagcc ttattttaac
      421 ttgctatttc tagctctaaa acgggctgca ggaattcgat ataagcttct gccaatcgca
      481 gctcccagag tttcgttccc agccgcgagc accacagcgt accatgtgcg ccacgaggcc
      541 tcaaacgtaa aacaatcgat acgaaaagaa acagactata ggggagtata gagacagccg
      601 gccaataaga
//
