LOCUS       sequence-112666-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR + ColE1 + PLlacO-1::RBS(st2):lacZ::RBS(st7):cfp::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 886
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    208..224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccaccttgtt catcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
       61 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      121 ccttaccaga gggcgcccca gctggcaatt ccgacgtctc gaactgcagg tcgactgatc
      181 cataaatgtg agcggataac attgacattg tgagcggata acaagatact ggtacctaag
      241 gaggaaatga ccatgattac ggattcactg gccgtcgtgg cccgcaccga tcgcccttcc
      301 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
      361 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
      421 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
      481 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
      541 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
      601 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
      661 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
      721 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
      781 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
      841 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
      901 gagttgcgtg actac
//
