LOCUS       sequence-112668-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + 
            PLlacO-1::RBS(st7):psu::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 1988
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(254..256,257..259,260..972)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDDCTS"
     primer_bind     complement(299..320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(560..579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     907..928
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccaccttg ttcatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
       61 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      121 aaccttacca gagggcgccc cagctggcaa ttccgacgtc tcgaactgca ggtcgactga
      181 tccataaatg tgagcggata acattgacat tgtgagcgga taacaagata ctggtaccta
      241 aggaggaaaa aaaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      301 ggtcgagctg gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg
      361 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      421 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      481 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      541 gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      601 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      661 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      721 caagcagaag aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag
      781 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      841 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
      901 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
      961 ctgtacaagt aaggatccta tgaagcttta atcgtacagg gtag
//
