LOCUS       sequence-112675-         356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  binary plant vector expressing GFP and GUS, both driven by a 35S 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Vickers CE, Schenk PM, Li D, Mullineaux PM, Gresshoff PM
  TITLE     pGFPGUSPlus, a new binary vector for gene expression studies and 
            optimising transformation systems in plants.
  JOURNAL   Biotechnol Lett. 2007 Nov;29(11):1793-6. Epub 2007 Aug 9.
  PUBMED    17687623
REFERENCE   2  (bases 1 to 356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett."; date: "2007-11"; volume: "29(11)"; pages: "1793-6. Epub 2007
            Aug 9"
COMMENT     Sequence Label: pGFPGUSPlus
FEATURES             Location/Qualifiers
     source          1..356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(277..294)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
       61 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
      121 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
      181 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
      241 cgatcgggga aattcgagct ggtcaccaat tcacacgtga tggtgatggt gatggctagc
      301 gttcttgtag ccgaaatctg gaatgttggt ccagcgctcg cgaaagacgt gcgcgg
//
