LOCUS       sequence-112677-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF promoter driven GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Williams TC, Espinosa MI, Nielsen LK, Vickers CE
  TITLE     Dynamic regulation of gene expression using sucrose responsive 
            promoters and RNA interference in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7.
  PUBMED    25886317
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-04-1"; volume: "14:43. doi"; pages: " 
            10.1186/s12934-015-0223-7"
COMMENT     Sequence Label: PTEF1-yEGFPCLN2PEST-pRS406
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(62..468)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(498..517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(499..517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(527..544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(527..543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaattgggac aacaccagtg aataattctt cacctttaga catgaattct tgtaattaaa
       61 acttagatta gattgctatg ctttctttct aatgagcaag aagtaaaaaa agttgtaata
      121 gaacaagaaa aatgaaactg aaacttgaga aattgaagac cgtttattaa cttaaatatc
      181 aatgggaggt catcgaaaga gaaaaaaatc aaaaaaaaaa attttcaaga aaaagaaacg
      241 tgataaaaat ttttattgcc tttttcgacg aagaaaaaga aacgaggcgg tctctttttt
      301 cttttccaaa cctttagtac gggtaattaa cgacacccta gaggaagaaa gaggggaaat
      361 ttagtatgct gtgcttgggt gttttgaagt ggtacggcga tgcgcggagt ccgagaaaat
      421 ctggaagagt aaaaaaggag tagaaacatt ttgaagctat ggtgtgtgcc tcgagggggg
      481 gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      541 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      601 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      661 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      841 gctcccttta gggttccgat ttagtgcttt a
//
