LOCUS       sequence-112679-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SUC2 regulated expression of an antisense RNAi construct for GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Williams TC, Espinosa MI, Nielsen LK, Vickers CE
  TITLE     Dynamic regulation of gene expression using sucrose responsive 
            promoters and RNA interference in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7.
  PUBMED    25886317
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-04-1"; volume: "14:43. doi"; pages: " 
            10.1186/s12934-015-0223-7"
COMMENT     Sequence Label: PSUC2-GFPantisense-CYC1t-pRS413
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(62..78)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(62..78)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      104..351
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(120..138)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(397..417)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(397..415)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(436..452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(436..452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(449..471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    460..476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(484..490,491..508,509..514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    529..550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(667..684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt tagacccggg
       61 ggatccacta gttctagaac aggccccttt tcctttgtcg atatcatgta attagttatg
      121 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
      181 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
      241 tttatatttc aaatttttct tttttttctg tacaaacgcg tgtacgcatg taacattata
      301 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg caagcttcgc
      361 agtttacact ctcatcgtga gctccagctt ttgttccctt tagtgagggt taattgcgcg
      421 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      481 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      541 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      601 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      841 ccataggctc cgcccccctg acgagcatca caa
//
