LOCUS       sequence-112681-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SUC2 regulated expression of an antisense RNAi construct for GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Williams TC, Espinosa MI, Nielsen LK, Vickers CE
  TITLE     Dynamic regulation of gene expression using sucrose responsive 
            promoters and RNA interference in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7.
  PUBMED    25886317
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-04-1"; volume: "14:43. doi"; pages: " 
            10.1186/s12934-015-0223-7"
COMMENT     Sequence Label: PSUC2-GFPantisense-CYC1t-pRS413
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..288)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     254..272
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     314..330
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     314..330
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aaagctggag ctcacgatga gagtgtaaac tgcgaagctt gcaaattaaa gccttcgagc
       61 gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac acgcgtttgt
      121 acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa
      181 aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg gttagagcgg
      241 atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgata tcgacaaagg
      301 aaaaggggcc tgttctagaa ctagtggatc ccccgggtct aaaggtgaag aattattcac
      361 tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt
      421 ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac
      481 tactggtaaa ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca
      541 atgttttgcg agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc
      601 agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag
      661 agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga
      721 ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa
      781 tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca
      841 caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg
      901 tgatggtc
//
