LOCUS       sequence-112687-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF promoter driven GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Williams TC, Espinosa MI, Nielsen LK, Vickers CE
  TITLE     Dynamic regulation of gene expression using sucrose responsive 
            promoters and RNA interference in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7.
  PUBMED    25886317
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-04-1"; volume: "14:43. doi"; pages: " 
            10.1186/s12934-015-0223-7"
COMMENT     Sequence Label: PTEF1-yEGFPCLN2PEST-pRS406
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(56..243)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     164..188
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccg atctgccggt
       61 agaggtgtgg tcaataagag cgacctcata ctatacctga gaaagcaacc tgacctacag
      121 gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt
      181 gtatacactt atttttttta taacttattt aataataaaa atcataaatc ataagaaatt
      241 cgcttattta gaagtggcgc gccctatatt acttgggtat tgcccatacc aaaagaaatt
      301 aaagatgagg cactgctaga tttaccgcta tttatggtcc cagttggcga ggggaacatt
      361 ccatggttaa tgaagcttgt cttgggtggg cccccattga ggtaatgcgc cgttgcggga
      421 ttttggtttt ccttgttaga ctcatttagg tttagcaaat caatataatc attggttgcg
      481 ttattgctgt taggacccgt gatttgttcg tagatccttt gtttgttatt tgtattcacc
      541 agactattca cactaacggt gctaccacat atactgtttg actgctgctg accaaattgg
      601 tacatacttg gagtgattgg tgatgattgt tccatattcc ggctgaaaac gctgttatca
      661 gagagtgagc tcatgtttct cattggagtg tggctttgag atgaggaagc gggcgaagga
      721 atggatgtgc tatttgaatt ttcgaaagag catgatgggg ttgatatggt aagctttctc
      781 gaaatgttca agttggatgc tttgtacaat tcatccatac ca
//
