LOCUS       sequence-112688-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  binary plant vector expressing GFP and GUS, both driven by a 35S 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Vickers CE, Schenk PM, Li D, Mullineaux PM, Gresshoff PM
  TITLE     pGFPGUSPlus, a new binary vector for gene expression studies and 
            optimising transformation systems in plants.
  JOURNAL   Biotechnol Lett. 2007 Nov;29(11):1793-6. Epub 2007 Aug 9.
  PUBMED    17687623
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett."; date: "2007-11"; volume: "29(11)"; pages: "1793-6. Epub 2007
            Aug 9"
COMMENT     Sequence Label: pGFPGUSPlus
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..557,558..560,561..563))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     238..257
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     497..518
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
       61 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      121 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      181 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      241 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      301 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      361 ccgtaggtga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      421 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      481 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      541 agctcctcgc ccttgctcac catgggatc
//
