LOCUS       sequence-112689-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing the calcium indicator GCaMP6 driven by 
            the muscle-specific promoter MHCK7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Madden L, Juhas M, Kraus WE, Truskey GA, Bursac N
  TITLE     Bioengineered human myobundles mimic clinical responses of skeletal 
            muscle to drugs.
  JOURNAL   eLife. 2015 Jan 9;4:e04885. doi: 10.7554/eLife.04885.
  PUBMED    25575180
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2015-01-9"; volume: "4:e04885. doi"; pages: " 
            10.7554/eLife.04885"
COMMENT     Sequence Label: pRRL-MHCK7-GCaMP6
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     606..625
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     865..886
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacgctcac ttcgctgtca
       61 tcatttgtac aaactcttcg tagtttacct gaccatcccc atcgatgtct gcttccctga
      121 tcatttcatc aacctcttca tctgttaact tctctccaag gtttgtcatc acgtggcgaa
      181 gctctgctgc actgatgtag ccattgccat ccttatcaaa cacaccgaac gcttctctaa
      241 tttcttcttc cgtgtccctg tatttcattt ttcttgccat cattgtcagg aactcaggga
      301 agtcgattgt gccgtcaccg tcggcatcta cttcattgat catgtcctgc agctctgctt
      361 ctgtggggtt ctgccccaga gaccgcatca ccgtccccag ctccttggtt gttattgtcc
      421 catccccgtc cttgtcaaat agggagaatt cctctttaaa ttctgcgatc tgctcttcag
      481 tcagttggtc cggcaggttg tactccagct tgtgccccag gatgttgccg tcctccttga
      541 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      601 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg atgtagcctt
      661 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      721 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      781 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctca ccctcgccgg
      841 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      901 cggtgaacag ctcctcgccc ttgctcacca tgctccctcc ggtac
//
