LOCUS       sequence-112697-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK047
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..126
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(154..181)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(197..268)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
       61 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      121 ttttattggt ctggtccagc tgagacctat aaacgcagaa aggcccaccc gaaggtgagc
      181 cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      241 ctttcgactg agcctttcgt tttatttgat gcctggtcat ttgtacagtt catccatacc
      301 atgcgtgatg cccgctgcgg ttacgaactc cagcagaacc atatgatcgc gtttctcgtt
      361 cggatcttta gacagaacgc tttgcgtgct cagatagtga ttgtctggca gcagaacagg
      421 accatcaccg attggagtgt tttgctggta gtgatcagcc agctgcacgc tgccatcctc
      481 cacgttgtgg cgaattttaa aattcgcttt aatgccattt ttttgtttat cggcggtgat
      541 gtaaacattg tggctgttaa aattgtattc cagcttatgg cccaggatat tgccgtcctc
      601 tttaaagtca atgcctttca gctcaatgcg gtttaccagg gtatcgcctt caaatttcac
      661 ttccgcacgc gttttgtacg tgccgtcatc cttaaaggaa atcgtgcgtt cctgcacata
      721 gccttccggc atggcggact tgaagaagtc atgctgcttc atatggtccg gataacgagc
      781 aaagcactga acaccataag tcagcgtcgt tac
//
