LOCUS       sequence-112713-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 4a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK058
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      100..134
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg
       61 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      121 cgggcgtttt ttattggtct ggtctcagcc actcgagtta tttgtacaat tcatccatac
      181 catgggtaat accagcagca gtaacaaatt ctaacaagac catgtgatct ctcttttcgt
      241 ttggatcttt ggataaggca gattgatagg ataagtaatg gttgtctggt aacaagactg
      301 gaccatcacc aattggagta ttttgttgat aatggtcagc taattgaaca ccaccatctt
      361 caatgttgtg tctaattttg aagttagctt tgataccatt cttttgtttg tcagcagtga
      421 tgtaaacatt gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccacctt
      481 ctttaaaatc aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga
      541 cttcagctct ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat
      601 aaccttctgg catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctag
      661 caaaacattg caaaccataa cctaaagtag tgactaaggt tggccatgga actggcaatt
      721 taccagtagt acaaatcaat tttaaggtca atttaccgta agtagcatca ccttcacctt
      781 cac
//
