LOCUS       sequence-112716-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for sgRNA and for Expression of Cas9 linked to BFP
            via T2A linked to Ad4 E1B via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..92
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tcactctcgg catggacgag ctgtacaagc tcgaggcaac aaacttctca ctactcaaac
       61 aagcaggtga cgtggaggag aatcccgggc ctcttaagat ggagagccga aatccttttc
      121 agcagggact gccagccggg ttcctgtctt catcctttgt ggagaatatg gaggtgccag
      181 cccccgagtg caacctgcga ctgctggcag gaaccgcagc tcgccacagt gaggatcctg
      241 aatcaccagc agccggcgga agtaggagag agagtgaatc acgaccagga ccttcaggag
      301 gaggagtcgc tgacctgttc ccagaactgc atcgcgtgct gacacgaagc tcctctggcc
      361 gagagagggg aatcaagaga gagcggcacg atgaaaccaa tcataggatt gagctgacag
      421 tggggctgat gtctcgcaaa cgacctgaga ctgtctggtg gtacgaagtg cagagcaccg
      481 gcacagacga agtgtccgtc atgcacgaga ggttttctct ggaacaggtg aagacctgtt
      541 ggctggagcc tgaagacgat tgggaggtgg ccatcagaaa ctacgccaag ctggctctga
      601 gaccagaccg gaagtacaag atcactaagc tgatcaacat ccggaatgct tgctatatca
      661 gcggcaatgg agcagaggtc g
//
