LOCUS       sequence-112718-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK060
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      93..127
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(154..171)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(175..246)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(441..460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc
       61 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
      121 tttttattgg tctggtctca gccactcgag ttagtgatga tgatgatgat gacctttatc
      181 atcatcgtcc ttataatctt tgtcatcatc atctttgtag tccttatcat catcgtcctt
      241 gtaatcggat tgagaccgac tacggttatc cacagaatca ggggataacg caggaaagaa
      301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccag
//
