LOCUS       sequence-112723-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for a sgRNA against the mouse Rosa26 locus and 
            Cas9 linked to BFP via a T2A peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
       61 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
      121 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
      181 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
      241 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
      301 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
      361 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
      421 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc
      481 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
      541 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga
      601 aggactggga ccctaagaag tacggcggct tcgacag
//
