LOCUS       sequence-112725-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the human AAVS1 locus for insertion of traffic 
            light reporter (TLR) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pAAVS1-TLR targeting vector
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..661
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ttagagcaga gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg
       61 tggacgagga agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa
      121 acccagaagc ccagagcagg gccttaggga agcgggaccc tgctctgggc ggaggaatat
      181 gtcccagata gcactgggga ctctttaagg aaagaaggat ggagaaagag aaagggagta
      241 gaggcggcca cgacctggtg aacacctagg acgcaccatt ctcacaaagg gagttttcca
      301 cacggacacc cccctcctca ccacagccct gccaggacgg ggctggctac tggccttatc
      361 tcacaggtaa aactgacgca cggaggaaca atataaattg gggactagaa aggtgaagag
      421 ccaaagttag aactcaggac caacttattc tgattttgtt tttccaaact gcttctcctc
      481 ttgggaagtg taaggaagct gcagcaccag gatcagtgaa acgcaccaga cagccgcgtc
      541 agagcagctc aggttctggg agagggtagc gcagggtggc cactgagaac cgggcaggtc
      601 acgcatcccc cccttccctc ccaccccctg ccaagctctc cctcccagga tcctctctgg
      661 ctccatcgta agcaaacctt agaggttctg gcaaggagag agatggct
//
