LOCUS       sequence-112726-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the human AAVS1 locus for insertion of traffic 
            light reporter (TLR) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pAAVS1-TLR targeting vector
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    196..697
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cgaattcgcc ctttgctttc tctgaccagc attctctccc ctgggcctgt gccgctttct
       61 gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc ctgccgcctc
      121 cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg ttgctgccca
      181 aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt cctctgagcg
      241 gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca accccatgcc
      301 gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca tctctcgttt
      361 cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct tcttgtaggc
      421 ctgcatcatc accgtttttc tggacaaccc caaagtaccc cgtctccctg gctttagcca
      481 cctctccatc ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac
      541 ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag tctgtgctag
      601 ctcttccagc cccctgtcat ggcatcttcc aggggtccga gagctcagct agtcttcttc
      661 ctccaacccg ggcccctatg tccacttcag gacagcatgt ttgctgcctc cagggatcct
      721 gtgt
//
