LOCUS       sequence-112727-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for repair of the traffic light reporter (TLR), providing the
            venus coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pTLR repair vector
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..68
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tagacgcgtt ggccacgaac ttctctctgt taaagcaagc aggagatgtt gaagaaaacc
       61 ccgggcctat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt
      121 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
      181 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
      241 ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc aaccacaccc
      301 agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
      361 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
      421 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc
      481 agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct gacggcggcc
      541 tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact
      601 tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
      661 atgtggacca cagactggaa agaatcaagg aggccgac
//
