LOCUS       sequence-112728-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean
            and tagged with 7X His at C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Dayananda KM, Gogia S, Neelamegham S
  TITLE     Escherichia coli-derived von Willebrand factor-A2 domain 
            fluorescence/Forster resonance energy transfer proteins that 
            quantify ADAMTS13 activity.
  JOURNAL   Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 
            10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.
  PUBMED    21146487
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2010.12.005"; journalName: "Anal Biochem"; date: 
            "2011-03-15- 15"; volume: "410"; issue: "2"; pages: "206-13"
COMMENT     Sequence Label: pRSET-XS-VWF
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..111
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(433..454)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
       61 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      121 caccccggtg aacagctcct cgcccttgct caccatgtta acgcagcacc tctgcagcac
      181 caggtcagga gcctctcggg ggagcgtctc aaagtcctgg atgaggatag gggcattggg
      241 ccagccaatc ctctccagct cctgcacgtt ggcattaggg cccactccaa tgggcaccac
      301 ctggatgtct ccaggcagcc tcttgatctc atcagaggca ggatttccgg tgaccatgta
      361 gaccaggttg ggcgcctgct cccgatcacc ctgaccggtc tcgtccatgc cgagagtgat
      421 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      481 gctcagcttg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      541 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg
      601 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      661 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      721 gatgcccttc agctcgatgc ggttcacca
//
