LOCUS       sequence-112729-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  controls the expression of S. pyogenes dCas9 from a Tc‐inducible 
            PTet promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-dCas9
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      82..109
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(221..240)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
       61 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataaatgg
      121 cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag
      181 agtcaattca gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      241 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      301 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      361 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      421 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      481 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      541 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      601 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttc
//
