LOCUS       sequence-112732-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tADH1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK063
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      95..129
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(184..371)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     292..316
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(566..585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
       61 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
      121 gttttttatt ggtctggtct cacagcgaaa tggggagcga tttgcaggca tttgctcggc
      181 atgccggtag aggtgtggtc aataagagcg acctcatgct atacctgaga aagcaacctg
      241 acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt
      301 taaaatttgt atacacttat tttttttata acttatttaa taataaaaat cataaatcat
      361 aagaaattcg cgccatgaga ccgactacgg ttatccacag aatcagggga taacgcagga
      421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacaga
//
