LOCUS       sequence-112735-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tPGK1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK064
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      95..129
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(566..585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
       61 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
      121 gttttttatt ggtctggtct cacagcacat agaaatatcg aatgggaaaa aaaaactgca
      181 taaaggcatt aaaagaggag cgaatttttt tttaataaaa atcttaataa tcattaaaag
      241 ataaataata gtctatatat acgtatataa ataaaaaata ttcaaaaaat aaaataaact
      301 attattttag cgtaaaggat ggggaaagag aaaagaaaaa aattgatcta tcgatttcaa
      361 ttcaattcaa tgccaggaga ccgactacgg ttatccacag aatcagggga taacgcagga
      421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
//
