LOCUS       sequence-112736-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tENO2 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK065
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      93..127
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(564..583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc
       61 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
      121 tttttattgg tctggtctca cagcattttt caaactgcaa attcaagaaa aagccacgcg
      181 tgtgcacctt ttttttcccc ttccagtgca ttatgcaata gacagcacga gtctttgaaa
      241 aagtaactta taaaactgta tcaattttta aacctaaata gattcataaa ctattcgtta
      301 atataaagtg ttctaaacta tgatgaaaaa ataagcagaa aagactaata attcttagtt
      361 aaaagcactg ccaggagacc gactacggtt atccacagaa tcaggggata acgcaggaaa
      421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcag
//
