LOCUS       sequence-112741-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted, FRET-based PKA activity reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Herbst KJ, Allen MD, Zhang J
  TITLE     Spatiotemporally regulated protein kinase A activity is a critical 
            regulator of growth factor-stimulated extracellular signal-regulated
            kinase signaling in PC12 cells.
  JOURNAL   Mol Cell Biol. 2011 Oct;31(19):4063-75. doi: 10.1128/MCB.05459-11. 
            Epub 2011 Aug 1.
  PUBMED    21807900
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.05459-11"; journalName: "Mol Cell Biol"; date: 
            "2011-10"; volume: "31"; issue: "19"; pages: "4063-75"
COMMENT     Sequence Label: pcDNA3-AKAR4-NES
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..103
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(join(155..169,170..178))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(182..214)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(218..235)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      247..256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(275..294)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(276..294)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(339..542)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(368..388)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
       61 cgctgaacct gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      121 tgaacagctc ctcgcccttg ctcaccatgg gatccttatc gtcatcgtcg tacagatccc
      181 gacccatttg ctgtccacca gtcatgctag ccataccatg atgatgatga tgatgagaac
      241 cccgcaccat ggtggcggcc gcaagcttgg gtctccctat agtgagtcgt attaatttcg
      301 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
      361 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
      421 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
      481 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
      541 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
      601 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
      661 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg t
//
