LOCUS       sequence-112749-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes LEU2 as a Type 6 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK075
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..126
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
       61 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      121 ttttattggt ctggtctcaa ctcctgccta tttaacgcca acgttgcgag atatacactc
      181 gaaatgtgtg atgtagatgt aaacagacgg ctatatgaat gccactagcc ggttatttta
      241 ctcctagtta atacatattt cctagaattt tttacgtgca taagagtaga atagtctaaa
      301 gagaagggtc ctccaggtta agcaaggatt ttcttaactt cttcggcgac agcatcaccg
      361 acttcggtgg tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc
      421 tttttaactg catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac
      481 atcattgcag cagacaagat agtggcgata gggttgacct tattctttgg caaatctgga
      541 gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc
      601 aaggacgcag atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg
      661 atatcaccaa acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact
      721 aggatcatgg cggcagaatc aatcaattga tgt
//
