LOCUS       sequence-11275-e       13753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Kessels HW, Kopec CD, Klein ME, Malinow R
  TITLE     Roles of stargazin and phosphorylation in the control of AMPA 
            receptor subcellular distribution.
  JOURNAL   Nat Neurosci. 2009 Jul . 12(7):888-96.
  PUBMED    19543281
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2009 Jul . 12(7):888-96."
COMMENT     Sequence Label: pSIN REP5-GFP-GluR2 (Q)
FEATURES             Location/Qualifiers
     source          1..13753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7646..7662
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7646..7662
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(8069..8071,8072..8782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8069..8071,8072..8782)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8111..8132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8372..8391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8719..8740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        11924..12028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12029..12097,12098..12889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12247..12266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13060..13648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13549..13568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagat ccgtttgtcg tgcaactgca
      121 aaaaagcttc ccgcaatttg aggtagtagc acagcaggtc actccaaatg accatgctaa
      181 tgccagagca ttttcgcatc tggccagtaa actaatcgag ctggaggttc ctaccacagc
      241 gacgatcttg gacataggca gcgcaccggc tcgtagaatg ttttccgagc accagtatca
      301 ttgtgtctgc cccatgcgta gtccagaaga cccggaccgc atgatgaaat acgccagtaa
      361 actggcggaa aaagcgtgca agattacaaa caagaacttg catgagaaga ttaaggatct
      421 ccggaccgta cttgatacgc cggatgctga aacaccatcg ctctgctttc acaacgatgt
      481 tacctgcaac atgcgtgccg aatattccgt catgcaggac gtgtatatca acgctcccgg
      541 aactatctat catcaggcta tgaaaggcgt gcggaccctg tactggattg gcttcgacac
      601 cacccagttc atgttctcgg ctatggcagg ttcgtaccct gcgtacaaca ccaactgggc
      661 cgacgagaaa gtccttgaag cgcgtaacat cggactttgc agcacaaagc tgagtgaagg
      721 taggacagga aaattgtcga taatgaggaa gaaggagttg aagcccgggt cgcgggttta
      781 tttctccgta ggatcgacac tttatccaga acacagagcc agcttgcaga gctggcatct
      841 tccatcggtg ttccacttga atggaaagca gtcgtacact tgccgctgtg atacagtggt
      901 gagttgcgaa ggctacgtag tgaagaaaat caccatcagt cccgggatca cgggagaaac
      961 cgtgggatac gcggttacac acaatagcga gggcttcttg ctatgcaaag ttactgacac
     1021 agtaaaagga gaacgggtat cgttccctgt gtgcacgtac atcccggcca ccatatgcga
     1081 tcagatgact ggtataatgg ccacggatat atcacctgac gatgcacaaa aacttctggt
     1141 tgggctcaac cagcgaattg tcattaacgg taggactaac aggaacacca acaccatgca
     1201 aaattacctt ctgccgatca tagcacaagg gttcagcaaa tgggctaagg agcgcaagga
     1261 tgatcttgat aacgagaaaa tgctgggtac tagagaacgc aagcttacgt atggctgctt
     1321 gtgggcgttt cgcactaaga aagtacattc gttttatcgc ccacctggaa cgcagacctg
     1381 cgtaaaagtc ccagcctctt ttagcgcttt tcccatgtcg tccgtatgga cgacctcttt
     1441 gcccatgtcg ctgaggcaga aattgaaact ggcattgcaa ccaaagaagg aggaaaaact
     1501 gctgcaggtc tcggaggaat tagtcatgga ggccaaggct gcttttgagg atgctcagga
     1561 ggaagccaga gcggagaagc tccgagaagc acttccacca ttagtggcag acaaaggcat
     1621 cgaggcagcc gcagaagttg tctgcgaagt ggaggggctc caggcggaca tcggagcagc
     1681 attagttgaa accccgcgcg gtcacgtaag gataatacct caagcaaatg accgtatgat
     1741 cggacagtat atcgttgtct cgccaaactc tgtgctgaag aatgccaaac tcgcaccagc
     1801 gcacccgcta gcagatcagg ttaagatcat aacacactcc ggaagatcag gaaggtacgc
     1861 ggtcgaacca tacgacgcta aagtactgat gccagcagga ggtgccgtac catggccaga
     1921 attcctagca ctgagtgaga gcgccacgtt agtgtacaac gaaagagagt ttgtgaaccg
     1981 caaactatac cacattgcca tgcatggccc cgccaagaat acagaagagg agcagtacaa
     2041 ggttacaaag gcagagcttg cagaaacaga gtacgtgttt gacgtggaca agaagcgttg
     2101 cgttaagaag gaagaagcct caggtctggt cctctcggga gaactgacca accctcccta
     2161 tcatgagcta gctctggagg gactgaagac ccgacctgcg gtcccgtaca aggtcgaaac
     2221 aataggagtg ataggcacac cggggtcggg caagtcagct attatcaagt caactgtcac
     2281 ggcacgagat cttgttacca gcggaaagaa agaaaattgt cgcgaaattg aggccgacgt
     2341 gctaagactg aggggtatgc agattacgtc gaagacagta gattcggtta tgctcaacgg
     2401 atgccacaaa gccgtagaag tgctgtacgt tgacgaagcg ttcgcgtgcc acgcaggagc
     2461 actacttgcc ttgattgcta tcgtcaggcc ccgcaagaag gtagtactat gcggagaccc
     2521 catgcaatgc ggattcttca acatgatgca actaaaggta catttcaatc accctgaaaa
     2581 agacatatgc accaagacat tctacaagta tatctcccgg cgttgcacac agccagttac
     2641 agctattgta tcgacactgc attacgatgg aaagatgaaa accacgaacc cgtgcaagaa
     2701 gaacattgaa atcgatatta caggggccac aaagccgaag ccaggggata tcatcctgac
     2761 atgtttccgc gggtgggtta agcaattgca aatcgactat cccggacatg aagtaatgac
     2821 agccgcggcc tcacaagggc taaccagaaa aggagtgtat gccgtccggc aaaaagtcaa
     2881 tgaaaaccca ctgtacgcga tcacatcaga gcatgtgaac gtgttgctca cccgcactga
     2941 ggacaggcta gtgtggaaaa ccttgcaggg cgacccatgg attaagcagc ccactaacat
     3001 acctaaagga aactttcagg ctactataga ggactgggaa gctgaacaca agggaataat
     3061 tgctgcaata aacagcccca ctccccgtgc caatccgttc agctgcaaga ccaacgtttg
     3121 ctgggcgaaa gcattggaac cgatactagc cacggccggt atcgtactta ccggttgcca
     3181 gtggagcgaa ctgttcccac agtttgcgga tgacaaacca cattcggcca tttacgcctt
     3241 agacgtaatt tgcattaagt ttttcggcat ggacttgaca agcggactgt tttctaaaca
     3301 gagcatccca ctaacgtacc atcccgccga ttcagcgagg ccggtagctc attgggacaa
     3361 cagcccagga acccgcaagt atgggtacga tcacgccatt gccgccgaac tctcccgtag
     3421 atttccggtg ttccagctag ctgggaaggg cacacaactt gatttgcaga cggggagaac
     3481 cagagttatc tctgcacagc ataacctggt cccggtgaac cgcaatcttc ctcacgcctt
     3541 agtccccgag tacaaggaga agcaacccgg cccggtcaaa aaattcttga accagttcaa
     3601 acaccactca gtacttgtgg tatcagagga aaaaattgaa gctccccgta agagaatcga
     3661 atggatcgcc ccgattggca tagccggtgc agataagaac tacaacctgg ctttcgggtt
     3721 tccgccgcag gcacggtacg acctggtgtt catcaacatt ggaactaaat acagaaacca
     3781 ccactttcag cagtgcgaag accatgcggc gaccttaaaa accctttcgc gttcggccct
     3841 gaattgcctt aacccaggag gcaccctcgt ggtgaagtcc tatggctacg ccgaccgcaa
     3901 cagtgaggac gtagtcaccg ctcttgccag aaagtttgtc agggtgtctg cagcgagacc
     3961 agattgtgtc tcaagcaata cagaaatgta cctgattttc cgacaactag acaacagccg
     4021 tacacggcaa ttcaccccgc accatctgaa ttgcgtgatt tcgtccgtgt atgagggtac
     4081 aagagatgga gttggagccg cgccgtcata ccgcaccaaa agggagaata ttgctgactg
     4141 tcaagaggaa gcagttgtca acgcagccaa tccgctgggt agaccaggcg aaggagtctg
     4201 ccgtgccatc tataaacgtt ggccgaccag ttttaccgat tcagccacgg agacaggcac
     4261 cgcaagaatg actgtgtgcc taggaaagaa agtgatccac gcggtcggcc ctgatttccg
     4321 gaagcaccca gaagcagaag ccttgaaatt gctacaaaac gcctaccatg cagtggcaga
     4381 cttagtaaat gaacataaca tcaagtctgt cgccattcca ctgctatcta caggcattta
     4441 cgcagccgga aaagaccgcc ttgaagtatc acttaactgc ttgacaaccg cgctagacag
     4501 aactgacgcg gacgtaacca tctattgcct ggataagaag tggaaggaaa gaatcgacgc
     4561 ggcactccaa cttaaggagt ctgtaacaga gctgaaggat gaagatatgg agatcgacga
     4621 tgagttagta tggattcatc cagacagttg cttgaaggga agaaagggat tcagtactac
     4681 aaaaggaaaa ttgtattcgt acttcgaagg caccaaattc catcaagcag caaaagacat
     4741 ggcggagata aaggtcctgt tccctaatga ccaggaaagt aatgaacaac tgtgtgccta
     4801 catattgggt gagaccatgg aagcaatccg cgaaaagtgc ccggtcgacc ataacccgtc
     4861 gtctagcccg cccaaaacgt tgccgtgcct ttgcatgtat gccatgacgc cagaaagggt
     4921 ccacagactt agaagcaata acgtcaaaga agttacagta tgctcctcca ccccccttcc
     4981 taagcacaaa attaagaatg ttcagaaggt tcagtgcacg aaagtagtcc tgtttaatcc
     5041 gcacactccc gcattcgttc ccgcccgtaa gtacatagaa gtgccagaac agcctaccgc
     5101 tcctcctgca caggccgagg aggcccccga agttgtagcg acaccgtcac catctacagc
     5161 tgataacacc tcgcttgatg tcacagacat ctcactggat atggatgaca gtagcgaagg
     5221 ctcacttttt tcgagcttta gcggatcgga caactctatt actagtatgg acagttggtc
     5281 gtcaggacct agttcactag agatagtaga ccgaaggcag gtggtggtgg ctgacgttca
     5341 tgccgtccaa gagcctgccc ctattccacc gccaaggcta aagaagatgg cccgcctggc
     5401 agcggcaaga aaagagccca ctccaccggc aagcaatagc tctgagtccc tccacctctc
     5461 ttttggtggg gtatccatgt ccctcggatc aattttcgac ggagagacgg cccgccaggc
     5521 agcggtacaa cccctggcaa caggccccac ggatgtgcct atgtctttcg gatcgttttc
     5581 cgacggagag attgatgagc tgagccgcag agtaactgag tccgaacccg tcctgtttgg
     5641 atcatttgaa ccgggcgaag tgaactcaat tatatcgtcc cgatcagccg tatcttttcc
     5701 actacgcaag cagagacgta gacgcaggag caggaggact gaatactgac taaccggggt
     5761 aggtgggtac atattttcga cggacacagg ccctgggcac ttgcaaaaga agtccgttct
     5821 gcagaaccag cttacagaac cgaccttgga gcgcaatgtc ctggaaagaa ttcatgcccc
     5881 ggtgctcgac acgtcgaaag aggaacaact caaactcagg taccagatga tgcccaccga
     5941 agccaacaaa agtaggtacc agtctcgtaa agtagaaaat cagaaagcca taaccactga
     6001 gcgactactg tcaggactac gactgtataa ctctgccaca gatcagccag aatgctataa
     6061 gatcacctat ccgaaaccat tgtactccag tagcgtaccg gcgaactact ccgatccaca
     6121 gttcgctgta gctgtctgta acaactatct gcatgagaac tatccgacag tagcatctta
     6181 tcagattact gacgagtacg atgcttactt ggatatggta gacgggacag tcgcctgcct
     6241 ggatactgca accttctgcc ccgctaagct tagaagttac ccgaaaaaac atgagtatag
     6301 agccccgaat atccgcagtg cggttccatc agcgatgcag aacacgctac aaaatgtgct
     6361 cattgccgca actaaaagaa attgcaacgt cacgcagatg cgtgaactgc caacactgga
     6421 ctcagcgaca ttcaatgtcg aatgctttcg aaaatatgca tgtaatgacg agtattggga
     6481 ggagttcgct cggaagccaa ttaggattac cactgagttt gtcaccgcat atgtagctag
     6541 actgaaaggc cctaaggccg ccgcactatt tgcaaagacg tataatttgg tcccattgca
     6601 agaagtgcct atggatagat tcgtcatgga catgaaaaga gacgtgaaag ttacaccagg
     6661 cacgaaacac acagaagaaa gaccgaaagt acaagtgata caagccgcag aacccctggc
     6721 gactgcttac ttatgcggga ttcaccggga attagtgcgt aggcttacgg ccgtcttgct
     6781 tccaaacatt cacacgcttt ttgacatgtc ggcggaggat tttgatgcaa tcatagcaga
     6841 acacttcaag caaggcgacc cggtactgga gacggatatc gcatcattcg acaaaagcca
     6901 agacgacgct atggcgttaa ccggtctgat gatcttggag gacctgggtg tggatcaacc
     6961 actactcgac ttgatcgagt gcgcctttgg agaaatatca tccacccatc tacctacggg
     7021 tactcgtttt aaattcgggg cgatgatgaa atccggaatg ttcctcacac tttttgtcaa
     7081 cacagttttg aatgtcgtta tcgccagcag agtactagaa gagcggctta aaacgtccag
     7141 atgtgcagcg ttcattggcg acgacaacat catacatgga gtagtatctg acaaagaaat
     7201 ggctgagagg tgcgccacct ggctcaacat ggaggttaag atcatcgacg cagtcatcgg
     7261 tgagagacca ccttacttct gcggcggatt tatcttgcaa gattcggtta cttccacagc
     7321 gtgccgcgtg gcggatcccc tgaaaaggct gtttaagttg ggtaaaccgc tcccagccga
     7381 cgacgagcaa gacgaagaca gaagacgcgc tctgctagat gaaacaaagg cgtggtttag
     7441 agtaggtata acaggcactt tagcagtggc cgtgacgacc cggtatgagg tagacaatat
     7501 tacacctgtc ctactggcat tgagaacttt tgcccagagc aaaagagcat tccaagccat
     7561 cagaggggaa ataaagcatc tctacggtgg tcctaaatag tcagcatagt acatttcatc
     7621 tgactaatac tacaacacca ccacctctag aactagtgga tcccccgggc tgcaggaatt
     7681 cggcacgagg tgcatgggag ggtgctgata ttcccagaca ccaggactac agcggcagct
     7741 cagctaaaaa ctgcattcag ccagtcctcg ggacttcggg agcagggaca ggacgcaagg
     7801 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt
     7861 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa
     7921 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact
     7981 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg
     8041 actgattttt ggtgtctctt ctgctagggt gagcaagggc gaggagctgt tcaccggggt
     8101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     8161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     8221 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     8281 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     8341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     8401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     8461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     8521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     8581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8641 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     8701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8761 cggcatggac gagctgtaca aggctagcaa cagcatacag ataggggggc tatttccaag
     8821 gggcgctgat caagaataca gtgcatttcg ggtagggatg gttcagtttt ccacttcgga
     8881 gttcagactg acaccccata tcgacaattt ggaggtagcc aacagtttcg cagtcaccaa
     8941 tgctttctgc tcccagtttt caagaggagt ctacgcaatt tttggatttt atgacaagaa
     9001 gtctgtaaat accatcacat cattctgtgg gacactccat gtgtccttca tcacacctag
     9061 cttcccaaca gatggcacac atccatttgt catccagatg cgacctgacc tcaaaggagc
     9121 actccttagc ttgattgagt actaccaatg ggacaagttc gcatacctct atgacagtga
     9181 cagaggctta tcaacactgc aagctgttct ggattctgct gcagagaaga agtggcaggt
     9241 gactgctatc aatgtgggga acatcaacaa tgacaagaaa gatgagacct acagatcgct
     9301 ctttcaagat ctggagttaa aaaaagaacg gcgtgtaatc ctggactgtg aaagggataa
     9361 agtaaatgac attgtggacc aggttattac cattggaaaa catgttaaag ggtaccatta
     9421 tatcattgca aatctgggat tcactgatgg ggacctgctg aaaattcagt ttggaggagc
     9481 aaatgtctct ggatttcaga ttgtagacta cgacgattcc ctggtgtcta aatttataga
     9541 aagatggtca acactggaag agaaagaata ccctggagca cacacagcga caattaagta
     9601 tacttcggcc ctgacgtatg atgctgtcca agtgatgact gaagcattcc gtaaccttcg
     9661 gaagcagagg attgaaatat cccggagagg aaatgcaggg gattgtttgg ccaacccagc
     9721 tgtgccctgg ggacaagggg tcgaaataga aagggccctc aagcaggttc aagttgaagg
     9781 cctctctgga aatataaagt ttgaccagaa tggaaaacga ataaactaca caattaacat
     9841 catggagctc aaaacaaatg gaccccggaa gattgggtac tggagtgaag tggataaaat
     9901 ggttgtcacc ctaactgagc tcccatcagg aaatgacacg tctgggcttg aaaacaaaac
     9961 tgtggtggtc accacaatat tggaatctcc atatgttatg atgaagaaaa atcatgaaat
    10021 gcttgaaggg aatgagcgtt acgagggcta ctgtgttgac ttagctgcag aaattgccaa
    10081 acactgtggg ttcaagtaca agctgactat tgttggggat ggcaagtatg gggccaggga
    10141 tgccgacacc aaaatttgga atggtatggt tggagagctt gtctacggga aagctgacat
    10201 tgcaattgct ccattaacta tcactctcgt gagagaagag gtgattgact tctccaagcc
    10261 cttcatgagt cttggaatct ctatcatgat caagaagcct cagaagtcca aaccaggagt
    10321 gttttccttt cttgatcctt tagcctatga gatctggatg tgcattgtgt ttgcctacat
    10381 tggggtcagt gtagttttat tcctggtcag cagatttagc ccctacgagt ggcacactga
    10441 ggaatttgaa gatggaagag aaacacaaag tagtgaatca actaatgaat ttgggatttt
    10501 taatagtctc tggttttcct tgggtgcctt tatgcagcag ggatgcgata tttcgccaag
    10561 atccctctct gggcgcattg ttggaggtgt gtggtggttc tttaccctga tcataatctc
    10621 ctcctacacg gctaacttag ctgccttcct gactgtagag aggatggtgt ctcccatcga
    10681 aagtgctgag gatctgtcta agcaaacaga aattgcttat ggaacattag actctggctc
    10741 cactaaagag tttttcagga gatctaaaat cgcagtgttt gataaaatgt ggacttatat
    10801 gaggagtgca gagccctctg tgtttgtgag gactaccgca gaaggagtag ccagagtccg
    10861 gaaatccaaa ggaaagtatg cctacttgct ggagtccaca atgaacgagt acatcgagca
    10921 gaggaagcct tgtgacacca tgaaagtggg aggaaacttg gattccaaag gctacggcat
    10981 cgccacacct aaaggatcct cattaggaaa tgcggttaac ctcgcagtac taaaactgaa
    11041 tgaacaaggc ctgttggaca aattgaaaaa caaatggtgg tacgacaaag gagagtgcgg
    11101 cagcggggga ggtgattcca aggaaaagac cagtgccctc agtctgagca acgttgctgg
    11161 agtattctac atccttgtcg ggggccttgg tttggcaatg ctggtggctt tgattgagtt
    11221 ctgttacaag tcaagggccg aggcgaaacg aatgaaggtg gcaaagaatc cacagaatat
    11281 taacccatct tcctcgcaga attcccagaa ttttgcaact tataaggaag gttacaacgt
    11341 atatggcatc gagagtgtta aaatttaggg gatgaccttg agtgatgtca tgaggagcaa
    11401 ggcaaggctg tcaattacag gaagtactgg agaaaatgga cgtgttatga ctccagaatt
    11461 tcccaaagca gtgcatgcag gccttgggcc caatgatccg accagcaaaa ctcgatgtac
    11521 ttccgaggaa ctgatgtgca taatgcatca ggctggtaca ttagatcccc gcttaccgcg
    11581 ggcaatatag caacactaaa aactcgatgt acttccgagg aagcgcagtg cataatgctg
    11641 cgcagtgttg ccacataacc actatattaa ccatttatct agcggacgcc aaaaactcaa
    11701 tgtatttctg aggaagcgtg gtgcataatg ccacgcagcg tctgcataac ttttattatt
    11761 tcttttatta atcaacaaaa ttttgttttt aacatttcaa aaaaaaaaaa aaaaaaaaaa
    11821 aaaaaaaaaa aaaaagggaa ttcctcgatt aattaagcgg ccgctcgagg ggaattaatt
    11881 cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    11941 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    12001 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    12061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    12121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    12181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    12241 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
    12301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    12361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    12421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    12481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    12541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    12601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    12661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    12721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    12781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    12841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    12901 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    12961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    13021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    13081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    13141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    13201 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    13261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    13321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    13381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    13441 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    13501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    13561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    13621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcgt atggacatat
    13681 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
    13741 ggggacacta tag
//
