LOCUS       sequence-112753-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes NourseothricinR as a Type 6 part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK078
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..126
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(144..341)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
       61 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      121 ttttattggt ctggtctcaa ctccagtata gcgaccagca ttcacatacg attgacgcat
      181 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      241 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      301 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag ttactttaag ggcaaggcat
      361 agacatatac aaggcctgtt caccgtcaga tgctgttcca tcatataaag cagtatctaa
      421 accacataat gtgaaaccca ttcttctata agcatggata gcaggggcgt taacattagt
      481 aacttctaac cataaatgac cggctcctct ctctcttgca aattctgtag ccaaacccat
      541 caaagctctt cctacaccat gacctctatg ctctggggcg acttctatat cttcaacggt
      601 caaccttcta ttccaacctg aataagaaac gactacaaaa ccagccaaat caccgtcatc
      661 accgtatgct acgaaagttc ttgaatctgg gtcaccatct tctccgtcgt cgctttcgtc
      721 atcaga
//
