LOCUS       sequence-112755-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant EGFP expression plasmid for the creation of a heteroduplex 
            DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see 
            notes in comments section..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Zhou B, Huang C, Yang J, Lu J, Dong Q, Sun LZ
  TITLE     Preparation of heteroduplex enhanced green fluorescent protein 
            plasmid for in vivo mismatch repair activity assay.
  JOURNAL   Anal Biochem. 2009 May 1;388(1):167-9. doi: 
            10.1016/j.ab.2009.02.020. Epub 2009 Feb 25.
  PUBMED    19248754
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.02.020"; journalName: "Anal Biochem"; date: 
            "2009-05-1- 1"; volume: "388"; issue: "1"; pages: "167-9"
COMMENT     Sequence Label: p189
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      678..687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(729..750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggagctctcg cataatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      601 cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
      661 cccgggatat cccggtcgcc accatggtga gcaatcgcga ggagctgttc accggggtgg
      721 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      781 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      841 agctgcccgt gccctagccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      901 gccgctaccc
//
