LOCUS       sequence-112756-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK080
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      90..124
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(142..339)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag
       61 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
      121 ttattggtct ggtctcaact ccagtatagc gaccagcatt cacatacgat tgacgcatga
      181 tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat
      241 ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa
      301 tgacaagttc ttgaaaacaa gaatcttttt attgtcagtt actttaatct tgttcttcag
      361 caacaaaatg aacacaattt ccagctggat ctctcaaagc aaattctcta ccccatggct
      421 gttcaccaat ctcggtcata gctggaccag aagcatctct gaagtttgta gatacaactt
      481 ctgaccattc agcatataat tcatctaaac ccctgaccca gacccaagcc aatgtattat
      541 cagggacaac ttgatcttgg actgctgaga taaataaagt aacgtcatcc ctgacaacac
      601 cagcaaaatc gtcctcaaca aagtctcttg agaatcctaa cctatctgtc caaaactcta
      661 cagcacctgc aacatcccta gctgttaaaa caggaacggc agatgttaat ttagccattt
      721 ttgtttatgt tcggatgtga tgtgagaact gtatcctagc aagattttaa aa
//
