LOCUS       sequence-112757-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK081
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..126
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     misc_feature    148..651
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
       61 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      121 ttttattggt ctggtctcat cgggacggat cgcttgcctg taacttacac gcgcctcgta
      181 tcttttaatg atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt
      241 atttggattt tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa
      301 taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca
      361 agaaaagcag attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag
      421 gattttcgtg tgtggttttc tacacagaca agatgaaaca attcggcatt aatacctgag
      481 agcaggaaga gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat
      541 cttcggaaaa caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt
      601 atatatttat attaaaaaat ttaaattata attattttta tagcacgtga tactctgaga
      661 ccgactacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      781 ccc
//
