LOCUS       sequence-11276-e        3742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Kessels HW, Kopec CD, Klein ME, Malinow R
  TITLE     Roles of stargazin and phosphorylation in the control of AMPA 
            receptor subcellular distribution.
  JOURNAL   Nat Neurosci. 2009 Jul . 12(7):888-96.
  PUBMED    19543281
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2009 Jul . 12(7):888-96."
COMMENT     Sequence Label: pSIN ReP5-GFP-GluR1
FEATURES             Location/Qualifiers
     source          1..3742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             128..139
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(270..272,273..983)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(270..272,273..983)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(312..333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(573..592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     920..941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcgactcta gaggatcccc cgggctgcag gaattcggca cgagctcggc tccccttcca
       61 agagaaacaa gagaaacctc acggaaggaa gggaggaagg aaagaagccg gcagagcatc
      121 aagaagaatc gaagggaggg gagggaagac caaatctatg gttggaccag ggcttctttt
      181 tcgccaatgt aaaaaggaat atgccgtaca tctttgcctt tttctgcacc ggttttctag
      241 gtgcggttgt gggtgccaat ttcgctaggg tgagcaaggg cgaggagctg ttcaccgggg
      301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      961 tcggcatgga cgagctgtac aaggctagcc ccaacaatat ccagataggg gggttatttc
     1021 caaaccaaca atcacaggaa catgcggctt ttaggtttgc tttgtcacaa ctcacggagc
     1081 cccccaagct gcttccccag atcgatattg tgaacatcag cgacacgttt gagatgactt
     1141 accgtttctg ttcccagttc tccaaaggag tctatgccat ctttggattt tatgaacgaa
     1201 ggactgtcaa catgctgacc tccttctgtg gggccctcca tgtgtgcttc attactccaa
     1261 gttttcctgt tgacacatcc aatcaatttg tccttcagct acgcccggaa ctacaggaag
     1321 ctctcattag cattatcgac cattacaagt ggcaaacctt tgtctacatt tatgatgctg
     1381 accggggcct gtcagtcctg cagagagtct tggatacagc cgcagagaag aactggcagg
     1441 taacggctgt caacattctg acaaccaccg aggaaggata ccggatgctc tttcaggacc
     1501 tggagaagaa aaaggagagg ctggtggtgg ttgactgtga atcagaacgc ctcaacgcca
     1561 tcctgggcca gatcgtgaag ctagaaaaga atggcatcgg gtaccactac atcctcgcca
     1621 atctgggctt catggacatt gacttaaata agttcaagga gagcggacgc aatgtgacag
     1681 gtttccagct ggtgaactac acagacacga tcccagccag aatcatgcag caatggagga
     1741 caagtgactc ccgagaccat accagggtgg actggaagag gccaaagtac acttctgctc
     1801 tcacctatga tggtgtcaag gtgatggctg aggccttcca aagcctgcgg aggcagagga
     1861 ttgacatatc ccgccggggg aatgctgggg actgtctggc taacccagct gtgccctggg
     1921 gtcaagggat cgacatccag agagccctgc agcaggtgcg cttcgaaggt ttgacaggaa
     1981 atgtgcagtt caacgagaaa gggcgccgga ccaattacac cctccacgtg atcgaaatga
     2041 aacatgatgg aatccgaaag attggttact ggaatgaaga cgataaattt gtccccgcag
     2101 ccaccgacgc tcaggctgga ggggacaact caagcgtcca gaataggacc tacatcgtca
     2161 ctactatcct cgaagatcct tacgtgatgc ttaaaaagaa tgccaaccag tttgagggca
     2221 atgaccgcta tgagggctac tgtgtggagc tggctgcaga gatcgccaag cacgtgggct
     2281 actcctaccg acttgagatt gtcagcgacg gcaaatatgg agcccgggat cccgacacaa
     2341 aggcttggaa tggcatggtg ggagaactgg tctatggaag agcagacgtg gctgtggctc
     2401 ccttgaccat aaccttggtc cgggaggaag tcatcgactt ctccaagcca ttcatgagtt
     2461 tgggaatctc cattatgatt aagaagccac agaagtccaa gccaggtgtc ttctcctttc
     2521 ttgacccttt ggcctatgag atctggatgt gtatagtgtt tgcctacatt ggagtgagcg
     2581 tcgtcctctt cctggtcagc cgtttcagcc cctacgaatg gcacagcgaa gagtttgaag
     2641 agggacgaga ccagacaacc agtgaccagt caaatgagtt tggcatattc aacagcctgt
     2701 ggttctccct gggggccttc atgcagcaag gatgtgacat ttcccccagg tccctgtccg
     2761 gacgcatcgt cggcggcgtc tggtggttct tcactttgat catcatctcc tcgtacacag
     2821 ccaacctggc tgccttcctg actgtggaga ggatggtgtc tcccattgag agtgcagagg
     2881 acctggcaaa gcagacggaa attgcttatg ggacattgga agcaggctcc actaaggagt
     2941 tcttcaggag atctaaaatc gctgtgtttg agaagatgtg gacatacatg aagtctgcag
     3001 aaccatccgt gtttgttcgg accacagagg aaggcatgat cagagtgaga aaatctaaag
     3061 gcaaatacgc ctacctcctg gagtccacca tgaatgagta tattgagcaa cgaaagccct
     3121 gtgacaccat gaaagtggga ggtaacttgg attccaaagg ctatggcatt gcgacaccca
     3181 aggggtccgc cctgagaaat ccagtaaacc tggcagtgtt aaaactgaac gagcaggggc
     3241 ttttggacaa attgaaaaac aaatggtggt acgacaaggg cgagtgcggc agcgggggag
     3301 gtgactccaa ggacaagacc agcgctttga gcctcagcaa tgtggcaggc gtgttctaca
     3361 tcctgattgg agggctggga ctggccatgc tggttgcctt aatcgagttc tgctacaaat
     3421 cccgtagcga gtcgaagcgg atgaagggtt tctgtttgat cccacagcaa tccatcaatg
     3481 aagccatacg gacatcgacc ctcccccgga acagtggggc aggagccagc ggaggaggcg
     3541 cagtggagag aatggccggg tggtcagcca ggacttcccc aagtccatgc aatccattcc
     3601 ctgcatgagt cacagttcag ggatgccctt gggagccaca ggattgtaac tggagcagac
     3661 aggaaccctg gggagcaggc tcaggcttcc acagccccat cccaagccct tcagtgccaa
     3721 aaaaaaaaaa aaaaaactcg ag
//
