LOCUS       sequence-112763-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK084
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(114..141)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(157..228)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
       61 gaattggtta attggttgta acacgcggcc gctcggtgag accgacttat aaacgcagaa
      121 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
      181 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggagat
      241 ccttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg
      301 tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc
      361 tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct
      421 ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt
      481 tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag
      541 ttagtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg
      601 gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc
      661 gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa
      721 ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta
      781 actttcag
//
