LOCUS       sequence-112764-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SpecR-ColE1 as a Type 8 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK085
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(105..132)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(148..219)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
       61 gtagtcggca aataagcggc cgctcggtga gaccgactta taaacgcaga aaggcccacc
      121 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
      181 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggaga tccttattaa
      241 gcaccggtgg agtgacgacc ttcagcacgt tcgtactgtt caacgatggt gtagtcttcg
      301 ttgtgggagg tgatgtccag tttgatgtcg gttttgtaag cacccggcag ctgaaccggt
      361 tttttagcca tgtaggtggt tttaacttca gcgtcgtagt gaccaccgtc tttcagtttc
      421 agacgcattt tgatttcacc tttcagagca ccgtcttccg ggtacatacg ttcggtggaa
      481 gcttcccaac ccatggtttt tttctgcata accggaccgt cggacgggaa gttagtacca
      541 cgcagtttaa ctttgtagat gaactcaccg tcttgcaggg aggagtcctg ggtaacggta
      601 acaacaccac cgtcttcgaa gttcataaca cgttcccatt tgaaaccttc cgggaaggac
      661 agtttcaggt agtccgggat gtcagccggg tgtttaacgt aagctttgga accgtactgg
      721 aactgcgggg acaggatgtc ccaagcgaac ggcagcggac caccttt
//
