LOCUS       sequence-112771-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK089
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(184..211)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(227..298)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
       61 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      121 aaaaggatct aggtgaagat cctttttgat aatcgcggcc gctcggtgag accgacttat
      181 aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg
      241 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
      301 gcctggagat ccttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc
      361 aacgatggtg tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc
      421 acccggcagc tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg
      481 accaccgtct ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg
      541 gtacatacgt tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc
      601 ggacgggaag ttagtaccac gcagtttaac t
//
