LOCUS       sequence-112774-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes KanR-ColE1 as a Type 8a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK090
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(117..144)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(160..231)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
       61 tcagaattgg ttaattggtt gtaacacgcg gccgctcggt gagaccgact tataaacgca
      121 gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca
      181 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctgga
      241 gatccttatt aagcaccggt ggagtgacga ccttcagcac gttcgtactg ttcaacgatg
      301 gtgtagtctt cgttgtggga ggtgatgtcc agtttgatgt cggttttgta agcacccggc
      361 agctgaaccg gttttttagc catgtaggtg gttttaactt cagcgtcgta gtgaccaccg
      421 tctttcagtt tcagacgcat tttgatttca cctttcagag caccgtcttc cgggtacata
      481 cgttcggtgg aagcttccca acccatggtt tttttctgca taaccggacc gtcggacggg
      541 aagttagtac cacgcagttt aactttgtag atgaactcac cgtcttgcag ggaggagtcc
      601 tgggtaacgg taacaacacc accgtcttcg aagttcataa cacgttccca tttgaaacct
      661 tccgggaagg acagtttcag gtagtccggg atgtcagccg ggtgtttaac gtaagctttg
      721 gaaccgtact ggaactgcgg ggacaggatg tcccaagcga acggcagcgg accacctttg
      781 gtaactttca gtttagcggt ctgagtacct tcgtacggac gaccttcacc ttcaccttcg
//
