LOCUS       sequence-112776-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SpecR-ColE1 as a Type 8a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK091
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(106..133)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(149..220)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
       61 ggtagtcggc aaataagcgg ccgctcggtg agaccgactt ataaacgcag aaaggcccac
      121 ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac
      181 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggag atccttatta
      241 agcaccggtg gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc
      301 gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg
      361 ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt
      421 cagacgcatt ttgatttcac ctttcagagc accgtcttcc gggtacatac gttcggtgga
      481 agcttcccaa cccatggttt ttttctgcat aaccggaccg tcggacggga agttagtacc
      541 acgcagttta actttgtaga tgaactcacc gtcttgcagg gaggagtcct gggtaacggt
      601 aacaacacca ccgtcttcga agttcataac acgttcccat ttgaaacctt ccgggaagga
      661 cagtttcagg tagtccggga tgtcagccgg gtgtttaacg taagctttgg aaccgtactg
      721 gaactgcggg gacaggatgt cccaagcgaa cggcagcgga ccacctttgg taactttca
//
