LOCUS       sequence-112783-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK095
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(172..199)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(215..286)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
       61 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      121 gtgaagatcc tttttgataa tcgcggccgc tgtatgagac cgacttataa acgcagaaag
      181 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      241 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggtcattt
      301 gtacagttca tccataccat gcgtgatgcc cgctgcggtt acgaactcca gcagaaccat
      361 atgatcgcgt ttctcgttcg gatctttaga cagaacgctt tgcgtgctca gatagtgatt
      421 gtctggcagc agaacaggac catcaccgat tggagtgttt tgctggtagt gatcagccag
      481 ctgcacgctg ccatcctcca cgttgtggcg aattttaaaa ttcgctttaa tgccattttt
      541 ttgtttatcg gcggtgatgt aaacattgtg gctgttaaaa ttgtattcca gcttatggcc
      601 caggatattg ccgtcctctt taaagtcaat gcctttcagc tcaatgcggt ttaccagggt
      661 atcgccttca aatttcactt ccgcacgcgt tttgtacgtg ccgtcatcct taaaggaaat
      721 cgtgcgttcc tgcacatagc cttccggcat ggcggacttg aagaagtcat gctgcttcat
      781 atggtccgga taacgagcaa agcactgaac accataagtc agcgtcgtta ccagagtcgg
      841 ccaaggaacc ggcagtttac cagtagtaca gatgaacttc agcgtcagtt taccatt
//
