LOCUS       sequence-112786-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK095
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             268..279
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgcccct tgagaccatg
      121 aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt
      181 gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac
      241 ggcaggttcg gatcttagct actagagaaa gaggagaaat actagatgcg taaaggcgaa
      301 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat
      361 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag
      421 ttcatctgta ctactggtaa actgccggtt ccttggccga ctctggtaac gacgctgact
      481 tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag
      541 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg
      601 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg
      661 aaaggcattg actttaaaga ggacggcaat atcctgggcc ataagctgga atacaatttt
      721 aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt
      781 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac
      841 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaa
//
