LOCUS       sequence-112794-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses only N terminus of RNF8, amino acids 1 to 140.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, 
            Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo 
            M
  TITLE     The scaffold protein WRAP53beta orchestrates the ubiquitin response 
            critical for DNA double-strand break repair.
  JOURNAL   Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114.
  PUBMED    25512560
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.246546"; journalName: "Genes Dev"; date: "2014-12-15- 
            15"; volume: "28"; issue: "24"; pages: "2726-38"
COMMENT     Sequence Label: p3xFLAG-CMV-RNF8-N-terminus(1-140)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(752..771)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcaatggggg agcccggctt
      121 cttcgtcaca ggagaccgcg ccggtggccg gagctggtgc ctgcggcggg tggggatgag
      181 cgccgggtgg ctgctgctgg aagatgggtg cgaggtgact gtaggacgag gatttggtgt
      241 cacataccaa ctggtatcaa aaatctgccc cctgatgatt tctcgaaacc actgtgtttt
      301 gaagcagaat cctgagggcc aatggacaat tatggacaac aagagtctaa atggtgtttg
      361 gctgaacaga gcgcgtctgg aacctttaag ggtctattcc attcatcagg gagactacat
      421 ccaacttgga gtgcctctgg aaaataagga gaatgcggag tatgaatatg aagttactga
      481 agaagactgg gagacaatat atccttgtct ttccccaaag aattaaggta ccagtcgact
      541 ctagaggatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
      601 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
      661 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
      721 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
      781 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
      841 cctcc
//
