LOCUS       sequence-112803-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses only N terminus of RNF8, amino acids 1 to 111.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, 
            Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo 
            M
  TITLE     The scaffold protein WRAP53beta orchestrates the ubiquitin response 
            critical for DNA double-strand break repair.
  JOURNAL   Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114.
  PUBMED    25512560
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.246546"; journalName: "Genes Dev"; date: "2014-12-15- 
            15"; volume: "28"; issue: "24"; pages: "2726-38"
COMMENT     Sequence Label: p3xFLAG-CMV-RNF8-N-terminus(1-111)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(544..609)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(639..738)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(668..688)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcca taccaccccc ctccacccca taatattata gaaggacacc tagtcagaca
       61 aaatgatgca acttaatttt attaggacaa ggctggtggg cactggagtg gcaacttcca
      121 gggccaggag aggcactggg gaggggtcac agggatgcca cccgggatcc tctagagtcg
      181 actggtacct taaggcactc caagttggat gtagtctccc tgatgaatgg aatagaccct
      241 taaaggttcc agacgcgctc tgttcagcca aacaccattt agactcttgt tgtccataat
      301 tgtccattgg ccctcaggat tctgcttcaa aacacagtgg tttcgagaaa tcatcagggg
      361 gcagattttt gataccagtt ggtatgtgac accaaatcct cgtcctacag tcacctcgca
      421 cccatcttcc agcagcagcc acccggcgct catccccacc cgccgcaggc accagctccg
      481 gccaccggcg cggtctcctg tgacgaagaa gccgggctcc cccattgaat tcgcggccgc
      541 aagcttgtca tcgtcatcct tgtaatcgat gtcatgatct ttataatcac cgtcatggtc
      601 tttgtagtcc atggttaatt ctgacggttc actaaacgag ctctgcttat atagacctcc
      661 caccgtacac gcctaccgcc catttgcgtc aacggggcgg ggttattacg acattttgga
      721 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
      781 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattgg tgtactgcca aa
//
