LOCUS       sequence-112804-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 base vector with transcriptional terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  49..214
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(334..420)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(523..617)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(645..661)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(646..662)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gattcattaa tgcagcgccc aaacataaca ggaagaaaaa tgccccgctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttggcctg caaggccttc gcgaggtacc
      241 gggcccaagc ttctcgagga attcctgcag cccgggggat ccactagtga gctcatgcat
      301 gatatcgtcc gggccgcaag ctcctagcgg cggatttgtc ctactcagga gagcgttcac
      361 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      421 atgcctcaag ctagagagtc attaccccag gcgtttaagg gcaccaataa ctgccttaaa
      481 aaaattacgc cccgccctgc cactcatcgc agtctagctt ggattctcac caataaaaaa
      541 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
      601 atctatcaac aggagtccaa gctcagctaa ttaagctagc ttatcgatac cgtcgacctc
      661 gaaccccacg cccctcttta atacgacggg caatttgcac ttcag
//
