LOCUS       sequence-112805-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             147..158
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     mobile_element  268..433
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            592..700
                     /note="incP origin of transfer"
ORIGIN
        1 ttcagcttca cggtgttgtg gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
       61 aactcgtggc cgttcacggt gccctccatg cgcaccttga agcgcatgaa ctcggtgatg
      121 acgttctcgg aggaggccat gcttaatttc tcctctttaa ttctagatgt gtgaaattgt
      181 tatccgctca caattgaatc taagtatcat tgttatccgc tcacaagtca acactctttt
      241 tgataaattt tctcgacctt gcaggccaac cagataagtg aaatctagtt ccaaactatt
      301 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
      361 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
      421 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tgggcgctgc attaatgaat
      481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      541 tgacccgctg cgctcggtcg ttcggctgcg gcgagcggta tcagagctta tcggccagcc
      601 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      661 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      721 ccaagg
//
