LOCUS       sequence-112806-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged Med25-VWA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pcDNA5-Flag-Med25-VWA
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(718..741)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(830..868)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 actcgatcga gcggccgcct aaacaggcag cacgagtccc cgaaccagca ccatgtgcct
       61 cgggtcctgg ctcacatctg tcggaggctg cagcggctcc agcaaggccg ggggggctgc
      121 cttctcaaac agaagccgaa gcgcaggcag cttccgggga gacacaatgg agaagtggat
      181 cccccgctcc ccaatctgct gcacaagatt ctcagttgtg catccagagt acgtggtgct
      241 ctcaacagca ggcaacaagt atgggggtga gttgcagatg aggaggcaga cccggtgcgt
      301 ctggccaatc tgctcgcgca tcttcttgaa gtcatcaaac agctgcaagg ctgtgctgag
      361 tccttccgcg atgaggctgc agctctcacc acccccgccc atgaacttaa tgccatcgag
      421 ccaggtgaca aactcatagg cgctgctggt gggagcgtga cattgtacgt aggactcggg
      481 agcgcagtcc actgtgttga acaccacgag gctgtactgg gtccccccat agtctccccc
      541 gaagtccgtc tcagcaggag gaccaccatt aaaatactcg atggccggga gcaggtagtg
      601 cttgcggagc ccctcgaagt agggtcccag gttggccgta ccctcaatca caaacaccac
      661 gtcggccacc acgctcccgg cgcgggccgg gccctcggac ccggggacca tctcgagctt
      721 gtcatcatcg tccttgtagt ctggcatgct agccagcttg ggtctcccta tagtgagtcg
      781 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      841 tatagacctc ccaccgtaca cgcctaccgc c
//
