LOCUS       sequence-112815-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-eyfp
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..240
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     225..241
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      269..363
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      466..552
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    598..645
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tggatgccct gcgtaagcag gtgtgggcgg acaataaagt cttaaactga acaaaataga
       61 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      121 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      181 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      241 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      301 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      361 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      421 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      481 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      541 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      601 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
      661 ctgatgtgct t
//
