LOCUS       sequence-112822-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of GFP under the CaMKII promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CaMKII-GFP
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(87..800,801..803,804..806))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(90..800,801..803,804..806))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(132..153)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     481..500
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     740..761
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      803..812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttatcgataa gcttgatatc gaattcttac ttgtacagct cgtccatgcc gagagtgatc
      121 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      181 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      241 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      301 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      361 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      421 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      481 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      541 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
      601 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
      661 caaatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
      721 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
      781 aacagctcct cgcccttgct caccatggtg gcggatcccc cgctgccccc agaactaggg
      841 gccactcgcc tgcccgtgct cctgagtgca aacggagaac cggcttgact gacgagcttg
      901 gggcttctga gcagggcact gtggctgctc acagcctcgc gagccttcgt cagcatccag
      961 gtccccatgg caaccacctc cgaaacgccc tgttcccgtt gccccggtaa ctgcctcccc
     1021 aacacctgcc tgcctttcac tttaaaacct gctcctcttt gccctggcct tatatacaca
     1081 atactgcagg actgtgtgg
//
